Effect of 2-deoxy- D- Glucose and Cyclophosphamide Mediated Modification of Induced Genetic Damage in Albino Mice

The micronucleus test (MNT) on mammalian bone marrow is widely accepted as a toxicological screening test used as a sensitive method for measuring genetic damage. The aim of the current study was to evaluate frequency of micronucleated polychromatic erythrocytes (MNPCEs) in the bone marrow of mice treated with 2-Deoxy -DGlucose (2DG) and cyclophosphamide (CPH). Seven groups (n=10) of animals were selected and test compounds was injected intraperitoneally. The mean MnPCE values are found a significant inhibition in micronuclei formation was noticed with Cyclophosphamide 125 mg/kg body weight dose (p < 0.001) to compare with 2 DG and control groups. When we use 2-DG in the MN analysis, it was observed that the 0.5 mg/kg MnPCE level was higher in treated mice as compared to controls. The 1.0 mg/kg MnPCE level was lower. The 2.0 mg/kg MnPCE level also was lower however; the differences were highly significant (p < 0.001). The combination of Cyclophosphamide and 2-Deoxy -DGlucose 0.5 mg/kg MnPCE level were significantly increased and 1 mg/kg body weight was significantly decreased, overall the combination results were showing (p < 0.001). This study revealed that mice MN assay can be used as a genotoxicological test-system since some methodological particularities were observed.